Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: REG Iα promotes cell migration, invasion, and EMT via EDNRB. (A) Cell migration was assessed by Transwell assay. (B) Matrigel‐coated Transwell assay assessed cell invasion. (C) Western blot analysis of EMT‐related markers, including the epithelial marker E‐Cadherin and mesenchymal markers N‐Cadherin and Vimentin. β‐actin was used as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: Migration, Transwell Assay, Western Blot, Marker, Control

Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: The REG Iα‐EDNRB axis promotes cell migration, invasion, and EMT via the Ca 2+ signaling pathway. (A) Transwell assay assessed cell migration. (B) Cell invasion was assessed by Matrigel‐coated Transwell assay. (C) EMT‐related protein levels (E‐Cadherin, N‐Cadherin, Vimentin) were detected by Western blot. β‐actin served as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: Migration, Transwell Assay, Western Blot, Control

Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: The REG Iα‐EDNRB‐Ca 2+ axis promotes tumor growth and EMT in vivo. (A) Representative images of the xenograft tumors and excised tumor tissues from the indicated groups. (B) Statistical analysis of the final tumor weights. (C) Tumor growth curves measuring tumor volume over time. (D) H&E staining showed pathological changes in tumor tissues. (E) TUNEL assay detected cell apoptosis in tumor tissues. (F) Cell proliferation in tumor tissues was shown by Ki67 IHC staining. (G) Expression of REG Iα (Immunofluorescence, upper row) and EDNRB (IHC, lower row) in tumor tissues. (H) Western blot analysis of REG Iα, EDNRB, and p‐CaMKII protein levels in tumor tissues. β‐actin was used as the loading control. (I) Western blot analysis of EMT‐related proteins (E‐Cadherin, N‐Cadherin, Vimentin) in tumor tissues. β‐actin was used as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing, Immunofluorescence, Western Blot, Control

Journal: bioRxiv

Article Title: Maternal exposure to prostaglandin e2 results in abnormal dendritic morphology in the cerebellum and related motor behaviour in mouse offspring

doi: 10.1101/2023.10.12.562077

Figure Lengend Snippet: A. Cerebellar isolates of Saline control and PGE2-exposed male and female mice were quantified for the expression levels of Spinophilin (B), β-Actin (B) and N-Cadherin (D). Protein expression was normalized to the house keeping gene GAPDH and then quantified relative to the expression in Saline male controls. All samples are pooled from 10 animals derived from at least 4 separate litters. Data are presented as mean fold change ± SD. Each graph represents is an average of 3 individual Western blot runs per condition.

Article Snippet: Protein samples were probed with polyclonal rabbit anti-spinophilin (Abcam, 1:1000, ab18561, Cambridge, MA, USA) overnight at 4°C, and anti-β-Actin (Abcam, 1:5000, ab6276, Cambridge, MA, USA), anti-N-cadherin (Abcam; 1:10000, ab76011, Cambridge, MA, USA), and anti-GAPDH (Abcam; 1:5000, ab8245, Cambridge, MA, USA) for 1h at room temperature.

Techniques: Expressing, Derivative Assay, Western Blot

Journal: bioRxiv

Article Title: Maternal exposure to prostaglandin e2 results in abnormal dendritic morphology in the cerebellum and related motor behaviour in mouse offspring

doi: 10.1101/2023.10.12.562077

Figure Lengend Snippet: Cerebellar samples of pooled Saline controls (C57) and PGE2-exposed male P30 mice and selected PGE2-exposed male mice were analysed for the expression of Spinophilin, β-Actin and N-Cadherin. Protein expression was normalized to the house keeping gene GAPDH and then quantified relative to the expression in the pooled Saline Male controls. Pooled samples derive from 10 animals from at least4 separate litters (as in ). Male 1 had the best performance in cerebellar behaviour across all PGE2 males, while Males 2 and 3 had the worst performance across all PGE2 males. Data are presented as mean fold change ± SD. Graphs for each protein are representative of 3 individual runs per condition.

Article Snippet: Protein samples were probed with polyclonal rabbit anti-spinophilin (Abcam, 1:1000, ab18561, Cambridge, MA, USA) overnight at 4°C, and anti-β-Actin (Abcam, 1:5000, ab6276, Cambridge, MA, USA), anti-N-cadherin (Abcam; 1:10000, ab76011, Cambridge, MA, USA), and anti-GAPDH (Abcam; 1:5000, ab8245, Cambridge, MA, USA) for 1h at room temperature.

Techniques: Expressing

Journal: BMC Cancer

Article Title: CDC6, a key replication licensing factor, is overexpressed and confers poor prognosis in diffuse large B-cell lymphoma

doi: 10.1186/s12885-023-11186-6

Figure Lengend Snippet: CDC6 regulates downstream signalings related to cell cycle and apoptosis. A and B, SUHDL4 cells ( A ) and OCI-LY7 ( B ) were transfected with pLenO vector (LV-NC), pLenO-CDC6 (LV-CDC6), pLenR-GPH vector (LV-shCtrl) and pLenR-shCDC6 (LV-shCDC6) for 72 h, respectively, and the levels of CDC6, caspase-3, Bcl-2, Bax and GAPDH were analyzed by Western blot analysis. C and D, the experiments were performed as described in A and B , the cell lysates were collected and analyzed by western blot with antibodies against INK4, E-Cadherin, ATR, and GAPDH, respectively

Article Snippet: The primary antibodies against CDC6 (ab109315), caspase-3 (ab197202), bcl-2 (ab182858), Bax (ab182734), E-cadherin (ab40772), and ATR (ab13798) were purchased from abcam, UK; anti-INK4 (Cst 74,560) was from Cell Signaling Technology, US.

Techniques: Transfection, Plasmid Preparation, Western Blot

Journal: Viruses

Article Title: E6/E7 Functional Differences among Two Natural Human Papillomavirus 18 Variants in Human Keratinocytes

doi: 10.3390/v13061114

Figure Lengend Snippet: Migration and invasion potential, and EMT phenotype of immortalized PHK18A1 and PHK18B1: ( A ) wound healing assays. Cells were seeded and a wound was made throughout the center of the well. Images illustrate representative wells from three independent experiments carried out in triplicate. Histograms show the average area covered 12 h after wounding; ( B ) invasion assay. Cells were plated in collagen-covered inserts in nonsupplemented KSFM and invasion was stimulated by adding KSFM 15% FBS outside the inserts. Cellular invasion was monitored for 72 h. Images are representative of three independent experiments conducted in triplicate. Histograms show the average absorbance at 540 nm. ** p = 0.02; ( C ) levels of proteins associated with epithelial–mesenchymal transition (EMT). Cells were seeded in low-attachment plates in triplicates. After 14 days, spheroids formed were disassembled and the levels of E-cadherin and vimentin assessed using flow cytometry. Percentage of E-cadherin and vimentin-positive cells are presented. The average of three experiments is shown. The T -test was used for statistical analysis. * p = 0.00, *** p = 0.03.

Article Snippet: Cells were incubated with PE-anti-E-cadherin (562870, BD Biosciences, San Jose, CA, USA) and Alexa Fluor 488-anti-vimentin (562338, BD Biosciences), or PE-Mouse IgG1-Isotype Control (554680, BD Biosciences,), and Alexa Fluor 488-Mouse IgG1 Isotype Control (557721, BD Biosciences) for 40 min in ice, followed by flow cytometry.

Techniques: Migration, Invasion Assay, Flow Cytometry